The following are resources to give background to the lectures and experiments for B M B 443W (Laboratory in Protein Purification and Enzymology), taught at University park by Reddy Padala (vrp2@psu.edu).

Please note, most of the links in this guide are to subscription based resources available to PSU students, staff and faculty. 

Lai CY, Tchola O, Cheng T, Horecker BL. 1965. The Mechanism of Action of Aldolases VIII. THE NUMBER OF COMBINING SITES IN FRUCTOSE DIPHOSPHATE ALDOLASE*. Journal of Biological Chemistry, 240:1347-1350. Print: QP501.J7 4th Floor Paterno

Avigad G, Englard S. 1972. The Covalent Binding of D-Fructose 1,6-Diphosphate to Muscle Aldolase. Archives of Biochemistry and Biophysics. 153:337-346. Please note, we no longer have PRINT copies of this volume.

Blonski C, Moissac D DE, Perie J, Sygusch J. 1997. Inhibition of rabbit muscle aldolase by phosphorylated aromatic compounds. Biochemical Journal, 323:71-77.  Print:  QP501.B47 4th Floor Paterno

Lobb RR, Stokes AM, Hill HAO, Riordan JF. 1976. A Functional Arginine Residue in Rabbit-Muscle Aldolase. European Journal of Biochemistry (now called FEBS Journal), 70:517-522. Print: QP501.E9 4th Floor Paterno

Hill HAO, Lobb RR, Sharp SL, Stokes AM, Harris JI, Jack RS. 1976. Metal-Replacement Studies in Bacillus stearothermophilus Aldolase and a Comparison of the Mechanisms of Class I and Class II Aldolases. Biochemical Journal, 153:551-560. Print: QP501.B47 4th Floor Paterno

Richards OC, Rutter WJ. 1961. Comparative properties of yeast and muscle aldolase. Journal of Biological Chemistry, 236:3185-3191. Print: QP501.J7 4th Floor Paterno

Penhoet EE, Rutter WJ. 1975. [38] Detection and isolation of mammalian fructose-diphosphate aldolases. Methods in Enzymology. 42:240-249. Print: QP601.C733 4th Floor Paterno

Susor WA, Penhoet E, Rutter WJ. 1975. [15] Fructose-diphosphate aldolase, pyruvate kinase, and pyridine nucleotide-linked activities after electrophoresis. Methods in Enzymology. 41:66-73. Print: QP601.C733 4th Floor Paterno

Macdonald JA, Storey KB. 2002. Purification and characterization of fructose bisphosphate aldolase from the ground squirrel, Spermophilus lateralis: enzyme role in mammalian hibernation. Archives of Biochemistry and Biophysics. 408:279-285. Print: QP501.A77 4th Floor Paterno

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding., Analytical Biochemistry. 72:248-254. Please note, we no longer have PRINT copies of this volume.

Weber K, Pringle JR, Osborn M. 1972. [1] Measurement of molecular weights by electrophoresis on SDS-acrylamide gel. Methods in Enzymology. 26:3-27. Print: QP601.C733 4th Floor Paterno

Laemmli UK. 1970. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature. 227:680-685.  Print: Q1.N2 Annex

Lai CY, Nakai N, Chang D. 1974. Amino Acid Sequence of Rabbit Muscle Aldolase and the Structure of the Active Center. Science. 183:1204–1206. Print: Q1.S35 4th Floor Paterno